2. What organism is this?

3. What are the names of the parts of the microscope labelled A, B, C?

4. T F A petri plate containing only agar and water would support bacterial growth very well because the agar is a carbohydrate.

5. T F The holding temperature for agar based media is 40C.

6. Based on colony size, shape and pigment, which bacteria might this be?

7. T F During the first step of the gram stain procedure only gram positive bacteria will pick up the purple stain.

8. What is the name of the counterstain used in the Gram stain?

9. T F Endospores are reproductive structures used by many bacteria during times of stress.

10.T F Acid fast bacteria are fixed to the slide using acid while non-acid fast bacteria will not fix to the slide.

11. What would the bacteria look like if the gram decolorizer was used in the acid-fast staining procedure?

12. What would the bacteria look like if the acid-fast decolorizer was used in the gram stain procedure?
 
13. The image of the specimen in the microscope is too dim. What would be adjusted to correct this?

14. In order to get an accurate count of the number of closely spaced flagella on a bacterium, would it be better to use a microscope that has a green source light (513 nm) or a microscope which uses a violet source light (425 nm)?

15. Which would result in the least bending of light, light moving from glass to air or light moving from glass to oil? How does this affect the resolution (hint: numerical aperture)?

16. What is the easiest way to crack a slide while viewing it?

17. Which specific ingredient(s) is/are often added to provide the bulk of the nutrition in a complex media.

18. What is the difference between morphology and arrangement?

19. Which reagent(s), if any, are found in common between the Gram stain, acid fast stain and endospore stain?

20. Which would be most likely to show brownian motion, a small Staphylococcus aureus or a large Saccharomyces cerevisiaea?