Microbiology 225 Sample Practical II Questions
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1. The catalase test is best used to differentiate between various species of Streptoccocus.

True
False

2. Blood agar and coagulase are used to differentiate Staphylococcus from Streptococcus.

True
False

3. pH indicator molecules, such as Litmus, change color with varying pH. This is very useful in showing fermentation that results in mixed acids.

True
False

4. If, during the nitrate reduction test, the reaction turns red after the addition of zinc, this means that only nitrite reductase is present.

True
False

5. Optochin is used to differentiate between

Streptoccoccus and Staphylococcus
Alpha hemolytic Streptoccoccus
Beta hemolytic Staphylococcus
Proteus and E. coli

6. In the Litmus Milk test, a brown color indicates a breakdown of

Litmus
Lactose
Casein
B and C

7. Carbohydrate fermentation may result in the production of

acidic products
non-acidic products
gas (CO2)
All of the above

8. Manitol Salt Agar is considered a

differential media only
selective media only
selective and differential media
none of the above

9. The functional location of exo-enzymes is

inside the bacteria
outside the bacteria
exogenous reticulum
none of the above

10. Staphylococcus aureus is a formidable pathogen and is usually poistive for: catalase, coagulase, manitol fermentation, DNAse and beta hemolysis.

True
False